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e.ebong@northeastern.edu

Faculty Profile

Eno E. Ebong

Assistant Professor, Chemical Engineering
Affiliated Faculty, Bioengineering
617.373.8744
214B Lake Hall
43 Leon Street
Boston, MA 02115

Biography

The primary focus of our research is to study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis. We are applying engineering to study the structure and function of the endothelial cell surface glycocalyx that directly interfaces with flowing blood and sheds in the presence of atherosclerosis. We are using cryopreservation (rapid freezing/freeze substitution) and transmission electron microscopy to define the ultrastructure of the endothelial surface glycocalyx and its changes as a result of the macro- or micro-vessel origin and due to the bio-chemical and -mechanical environment. RNA interference techniques, fluorescent intracellular biomarkers, fluorescence confocal microscopy, and protein biochemistry are applied to further clarify the mechanisms by which various flow patterns impact endothelial cell surface glycocalyx ultrastructure, its transduction of fluid forces into biological responses, and its role in vascular health or disease. In vivo studies are performed using high fat fed apolipoprotein E (ApoE) knockout mice, a well-established animal model of atherosclerosis, to determine which glycocalyx components can be targeted to prevent, diagnose, or treat atherosclerosis.

Education

  • B.S. (Mechanical Engineering) Massachusetts Institute of Technology, 1999
  • M.Eng. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2001
  • Ph.D. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2006
  • Postdoctoral Fellowship Albert Einstein College of Medicine and CUNY City College of New York, 2007-2012

Research & Scholarship Interests

Study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis
Affiliated With

Department Research Areas

Honors & Awards

  • NIH Mentored Research Scientist Career Development Award

Selected Publications

  • E. Ebong, S.V. Lopez-Quintero, V. Rizzo, D.C. Spray, J.M.Tarbell, Shear-induced Endothelial NOS Activation and Remodeling via Heparin Sulfate, Glypican-1, and Syndecan-1, Integrative Biology: Quantitative Biosciences from Nano to Macro, 6(3), 2014, 338-347
  • M. Thi, E. Ebong, D. Spray, S. Suadicani, Interaction of the Glycocalyx with the Actin Cytoskeleton, Neuromethods, Springer Publishing, 79, 2013, 43-62
  • E. Ebong, N. Depaola, Specificity in the Participation Of Connexin Proteins in Flow-Induced Endothelial gap Junction Communication, European Journal of Physiology, 465(9), 2013, 1293-302
  • Y. Zeng, E. Ebong, B. Fu, J. Tarbell, The Structural Stability of the Endothelial Glycocalyx after Enzymatic Removal of Glycosaminoglycans, PLoS ONE, 7(8), 2012, e43168
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy, Arteriosclerosis Thrombosis and Vascular Biology, 31(8), 2011, 1908-1915
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Life-like Preservation and TEM Visualization of the Glycocalyx Reveals that it is Substantial in Vitro, Proceedings of the 2011 IEEE 37th Annual Northeast Bioengineering Conference, 2011, 1-2

Related News

April 4, 2016

Solomon Mensah is a Doctoral student in the Department of Bioengineering and a 2016 recipient of the prestigious National Science Foundation Graduate Research Fellowship Award. He will use his award...

March 22, 2016

23 COE faculty and affiliates were recipients of FY17 TIER 1 Interdisciplinary Research Seed Grants for 12 different projects representing over $600K dollars of investment in research.

March 3, 2016

ChE Assistant Professor Eno Ebong presented an invited talk on “Biomechanics of Vascular Disease" at Tufts University.

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