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e.ebong@northeastern.edu

Faculty Profile

Eno E. Ebong

Assistant Professor, Chemical Engineering
Affiliated Faculty, Bioengineering
617.373.8744
214B Lake Hall
43 Leon Street
Boston, MA 02115

Biography

Joined the Chemical Engineering Department in Fall 2013.

The primary focus of our research is to study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis. We are applying engineering to study the structure and function of the endothelial cell surface glycocalyx that directly interfaces with flowing blood and sheds in the presence of atherosclerosis. We are using cryopreservation (rapid freezing/freeze substitution) and transmission electron microscopy to define the ultrastructure of the endothelial surface glycocalyx and its changes as a result of the macro- or micro-vessel origin and due to the bio-chemical and -mechanical environment. RNA interference techniques, fluorescent intracellular biomarkers, fluorescence confocal microscopy, and protein biochemistry are applied to further clarify the mechanisms by which various flow patterns impact endothelial cell surface glycocalyx ultrastructure, its transduction of fluid forces into biological responses, and its role in vascular health or disease. In vivo studies are performed using high fat fed apolipoprotein E (ApoE) knockout mice, a well-established animal model of atherosclerosis, to determine which glycocalyx components can be targeted to prevent, diagnose, or treat atherosclerosis.

Education

  • B.S. (Mechanical Engineering) Massachusetts Institute of Technology, 1999
  • M.Eng. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2001
  • Ph.D. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2006
  • Postdoctoral Fellowship Albert Einstein College of Medicine and CUNY City College of New York, 2007-2012

Research & Scholarship Interests

Study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis
Affiliated With

Department Research Areas

Honors & Awards

  • NIH Mentored Research Scientist Career Development Award

Selected Publications

  • M.J. Cheng, P. Prabakaran, R. Kumar, S. Sridhar, E.E. Ebong. Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery. Journal of Visualized Experiments. 2018 Jan; (131), e56760
  • R. Mitra, G.L. O'Neil, I.C. Harding, M.J. Cheng, S.A. Mensah, E.E. Ebong. Glycocalyx in Atherosclerosis-Relevant Endothelium Function and as a Therapeutic Target. Current Atherosclerosis Reports. 2017 Nov 10; 19(12): 63
  • S.A. Mensah, M.J. Cheng, H. Homayoni, B.D. Plouffe, A.J. Coury, E.E. Ebong. Regeneration of glycocalyx by heparan sulfate and sphingosine 1-phosphate restores inter-endothelial communication. PLoS One. 2017 Oct 12;12(10):e0186116
  • S. Russell-Puleri, N.G. dela Paz, D. Adams,  M. Chattopadhyay, L.E. Cancel, E. Ebong, A.W. Orr, J.A. Frangos, J.M. Tarbell. Fluid shear stress induces upregulation of COX-2 and PGI(2) release in endothelial cells via a pathway involving PECAM-1, PI3K, FAK, and p38. American Journal of Physiology – Heart and Circulatory Physiology. 2017 Mar 1; 312 (3): H485-H500
  • W.T. Wong, S. Ma, X.Y. Tian, A.B. Gonzalez, E.E. Ebong, H. Shen. Targeted Delivery of Shear Stress-Inducible Micrornas by Nanoparticles to Prevent Vulnerable Atherosclerotic Lesions. Methodist Debakey Cardiovasc J. 2016 Sep;12(3):152-156
  • L.M. Cancel*, E.E. Ebong*, S. Mensah, C. Hirschberg, J.M. Tarbell. Endothelial Glycocalyx, Apoptosis and Inflammation in an Atherosclerotic Mouse Model. Atherosclerosis. Atherosclerosis. 2016 Sep; 252:136-46 (* authors made equal contributions to this work)
  • M. Cheng, R. Kumar, S. Sridhar, T.J. Webster, E.E. Ebong. Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting. International Journal of Nanomedicine. 2016 Jul 21;11: 3305-15
  • E. Ebong, S.V. Lopez-Quintero, V. Rizzo, D.C. Spray, J.M.Tarbell, Shear-induced Endothelial NOS Activation and Remodeling via Heparin Sulfate, Glypican-1, and Syndecan-1, Integrative Biology: Quantitative Biosciences from Nano to Macro, 6(3), 2014, 338-347
  • M. Thi, E. Ebong, D. Spray, S. Suadicani, Interaction of the Glycocalyx with the Actin Cytoskeleton, Neuromethods, Springer Publishing, 79, 2013, 43-62
  • E. Ebong, N. Depaola, Specificity in the Participation Of Connexin Proteins in Flow-Induced Endothelial gap Junction Communication, European Journal of Physiology, 465(9), 2013, 1293-302
  • Y. Zeng, E. Ebong, B. Fu, J. Tarbell, The Structural Stability of the Endothelial Glycocalyx after Enzymatic Removal of Glycosaminoglycans, PLoS ONE, 7(8), 2012, e43168
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy, Arteriosclerosis Thrombosis and Vascular Biology, 31(8), 2011, 1908-1915
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Life-like Preservation and TEM Visualization of the Glycocalyx Reveals that it is Substantial in Vitro, Proceedings of the 2011 IEEE 37th Annual Northeast Bioengineering Conference, 2011, 1-2

Related News

March 26, 2018

BioE PhD student Solomon Mensah and CEO of Therapeutic Innovation was selected through a competitive process to present his Social Impact Pitch on "Re-Examining the Design of the Neonatal Bubble-CPAP for Application in the Developing World" at the 15th annual Global Health & Innovation Conference.

July 24, 2017

The Methodist DeBakey Cardiovascular Journal (MDCVJ) reports on leading edge research, diagnosis, and treatments from specialists at the Houston Methodist DeBakey Heart & Vascular Center and...

September 19, 2016

Eno Ebong, Prof. Abby Koppes, and Prof. Ryan Koppes were selected as three out of the 29 junior faculty members chosen to participate in the Tufts CTSI 2016-2017 Junior Faculty Research Career Development Forum.

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