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e.ebong@neu.edu

Faculty Profile

Eno E. Ebong

Assistant Professor, Chemical Engineering
Affiliated Faculty, Bioengineering
617.373.8744
214B Lake Hall
43 Leon Street
Boston, MA 02115

Biography

The primary focus of our research is to study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis. We are applying engineering to study the structure and function of the endothelial cell surface glycocalyx that directly interfaces with flowing blood and sheds in the presence of atherosclerosis. We are using cryopreservation (rapid freezing/freeze substitution) and transmission electron microscopy to define the ultrastructure of the endothelial surface glycocalyx and its changes as a result of the macro- or micro-vessel origin and due to the bio-chemical and -mechanical environment. RNA interference techniques, fluorescent intracellular biomarkers, fluorescence confocal microscopy, and protein biochemistry are applied to further clarify the mechanisms by which various flow patterns impact endothelial cell surface glycocalyx ultrastructure, its transduction of fluid forces into biological responses, and its role in vascular health or disease. In vivo studies are performed using high fat fed apolipoprotein E (ApoE) knockout mice, a well-established animal model of atherosclerosis, to determine which glycocalyx components can be targeted to prevent, diagnose, or treat atherosclerosis.

Education

  • B.S. (Mechanical Engineering) Massachusetts Institute of Technology, 1999
  • M.Eng. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2001
  • Ph.D. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2006
  • Postdoctoral Fellowship Albert Einstein College of Medicine and CUNY City College of New York, 2007-2012

Research & Scholarship Interests

Study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis
Affiliated With

Department Research Areas

Selected Publications

  • E. Ebong, S.V. Lopez-Quintero, V. Rizzo, D.C. Spray, J.M.Tarbell, Shear-induced Endothelial NOS Activation and Remodeling via Heparin Sulfate, Glypican-1, and Syndecan-1, Integrative Biology: Quantitative Biosciences from Nano to Macro, 6(3), 2014, 338-347
  • M. Thi, E. Ebong, D. Spray, S. Suadicani, Interaction of the Glycocalyx with the Actin Cytoskeleton, Neuromethods, Springer Publishing, 79, 2013, 43-62
  • E. Ebong, N. Depaola, Specificity in the Participation Of Connexin Proteins in Flow-Induced Endothelial gap Junction Communication, European Journal of Physiology, 465(9), 2013, 1293-302
  • Y. Zeng, E. Ebong, B. Fu, J. Tarbell, The Structural Stability of the Endothelial Glycocalyx after Enzymatic Removal of Glycosaminoglycans, PLoS ONE, 7(8), 2012, e43168
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy, Arteriosclerosis Thrombosis and Vascular Biology, 31(8), 2011, 1908-1915
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Life-like Preservation and TEM Visualization of the Glycocalyx Reveals that it is Substantial in Vitro, Proceedings of the 2011 IEEE 37th Annual Northeast Bioengineering Conference, 2011, 1-2

Related News

November 6, 2015

Chemical engineering has a record number of presenters at the 2015 AIChE Annual Meeting which will be held in Salt Lake City, Utah. Title Presenting Author Advisor Oral Presentations Monday, November...

September 18, 2015

ChE Assistant Professor Eno Ebong is using interdisciplinary research to search for the cause and treat­ment of vas­cular dis­eases that cause heart attacks and strokes.

September 10, 2015

Chemical engineering has a record number of presenters at the 2015 Biomedical Engineering Society Annual meeting which will be taking place in Tampa, FL from Oct 7-10. Podium Thursday, October 8...